Identification of differentially expressed proteins involved in fetal scarless wound healing using a rat model of cleft lip

In early pregnancy, fetal skin wounds can heal quickly and undergo a transition period from scarless healing to scar formation. The aim of the present study was identify potential biomarkers associated with repair cleft lips, in order determine intrinsic factors leading formation embryonic tissue. A stable model lip established using microsurgery by constructing wedge?shaped lip?like defect rats at gestational age (GA) 16.5 GA18.5. GA16.5 GA18.5 groups were used formation, respectively. fetuses returned uterus following surgery, then removed 72 h after procedure. Macroscopic observation histological examination carried out. Reverse transcription?quantitative (RT?q) PCR parallel reaction monitoring (PRM) detect mRNA protein expression levels, upper?left completely healed surgery group rats. However, this not case group. Histological indicated new follicles visible under epidermis (GA16.5). Scarring on wound some growth pro?inflammatory factors, including TNF??, also different between two groups. Label?free quantification identified differentially expressed proteins five (Smad4, Fabp5, S100a4, S100a8 S100a9) identified. relative these molecules levels measured RT?qPCR PRM. These may represent for rat wounds.